BIOACCUMULATION OF Pb(II) FROM AQUEOUS SOLU- TIONS BY Bacillus Cereus
نویسنده
چکیده
ABSTRACT Because of the severity of heavy metal contamination and potential adverse health impact on the public, tremendous efforts have been made to purify waters containing toxic metal ions. Biosorption is presented as an alternative to traditional physicochemical means for removing toxic metals from groundwaters and wastewaters. Removal of lead from solution was studied using growing cells and washed cells of Bacillus Cereus M16. The removal of Pb(II) ions with growing cells was maximum (85%) when initial lead concentration was 50 mg/L. Other process conditions were optimized. These were volume of medium: 40 ml in a 250-ml Erlenmeyer fl ask, temperature: 30oC, pH: 6.0, fermentation time: 30 hours, and inoculum concentration (24-hour cell growth): 4%. Biosorption of Pb(II) on washed biomass of the selected strain was investigated in batch mode and optimum conditions were determined. The uptake of metal was very fast, and equilibrium was attained within 30 minutes. It was found that the overall adsorption process was best described by pseudo second-order kinetics. Both Langmuir and Freundlich isotherms were tested, and it was found that the latter had a better fi t with the data. The adsorption continuously increased in the pH range of 3.6 – 6.0, beyond which the adsorption could not be carried out due to precipitation of metal. The highest value of lead uptake was 96%, with 1.8 g/L washed biomass (dry basis) at 20oC and 92% at 30oC.
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